Related Experiment Video
Updated: May 7, 2026

Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
Published on: April 16, 2014
Confocal imaging and three-dimensional visualization of thick autofluorescent specimens
Angela V Klaus1, Valerie Schawaroch, Kevin J Frischmann
1Department of Biology, Seton Hall University, South Orange, NJ, USA.
Abstract:
Three-dimensional (3-D) rendering methods (maximum intensity projection, alpha blending, and isosurface rendering) are described for the visualization of thick, autofluorescent, arthropod cuticular structures (e.g., Drosophila melanogaster external genitalic structures) imaged by confocal laser scanning microscopy (CLSM). Additionally, specimen mounting and data collection strategies for thick specimens are described. Axial aberration artifacts are discussed in the context of these methods because of the critical roles they play in the quality of final 3-D images.
Related Concept Videos
Three-Dimensional Microscopy in Microbiology
Confocal Fluorescence Microscopy
Super-resolution Fluorescence Microscopy
Two-Dimensional Microscopy in Microbiology
Immunofluorescence Microscopy
The...

