Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative Colorimetric Oligonucleotide Sensing using Gold Nanoparticles and Toluidine Blue O: Insights from Representative Viral Sequences.

Macromolecular bioscience·2026
Same author

Combined resistive switching memory and multi-state operation in terpyridine-based Pd(II) and Fe(III) complexes for neuromorphic applications.

Nanoscale·2026
Same author

Epitaxial β-Ga<sub>2</sub>O<sub>3</sub>/GaN Heterojunction Based UV-C/UV-A Photodetectors with Superior Responsivity and Stability Under Vertical and Lateral Mode Operations.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Growth and biological responses of soybean cultivars to gamma-ray and electron beam irradiation.

International journal of radiation biology·2026
Same author

Water-Processable PVA-SbQ as an Eco-Friendly Photoreversible Dual-Tone Photoresist for Advanced Lithography.

Chemistry, an Asian journal·2026
Same author

MXene-Mediated Internal Electric Field in WO<sub>3</sub>/AgBr Nanocomposites Enhances Visible-Light-Driven Peroxymonosulfate-Activation and Dual-Mode Antibacterial Performance.

Small methods·2025

Related Experiment Video

Updated: May 7, 2026

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
10:45

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

Local pH-responsive diazoketo-functionalized photoresist for multicomponent protein patterning.

Je Moon Yun1, Ramakrishnan Ganesan, Jae-Hak Choi

  • 1Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST) , Yuseong-gu, Daejeon 305-701, Korea.

ACS Applied Materials & Interfaces
|September 24, 2013
PubMed
Summary

This study introduces a pH-responsive photoresist for mild biomolecule immobilization. The novel material enables selective surface patterning of proteins like streptavidin for biosensor applications.

More Related Videos

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement
08:36

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Published on: September 6, 2011

Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium
12:38

Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium

Published on: December 16, 2011

Related Experiment Videos

Last Updated: May 7, 2026

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
10:45

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement
08:36

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Published on: September 6, 2011

Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium
12:38

Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium

Published on: December 16, 2011

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Biotechnology

Background:

  • Selective surface immobilization of biomolecules under mild conditions is crucial for biosensors and biotechnology.
  • Existing methods often struggle with maintaining biomolecular integrity during immobilization processes.

Purpose of the Study:

  • To develop a biocompatible and pH-responsive photoresist for controlled biomolecule patterning.
  • To enable photolithographic processes in localized pH ranges to prevent biomolecular denaturation.

Main Methods:

  • Synthesized a photoresist using diazoketo-functionalized methacrylate, methacrylic acid, and poly(ethylene glycol) methacrylate monomers.
  • Utilized pH-dependent solubility switching (insoluble at pH ≤ 6.4, soluble at pH ≥ 7.9).
  • Applied UV exposure to generate carboxylic acid groups via Wolff rearrangement, enabling dissolution of exposed regions at pH 6.4.

Main Results:

  • Demonstrated dual streptavidin patterning using distinct pH buffers (6.4 and 7.9).
  • Confirmed the stability of diazoketo groups in unexposed regions through multiple wet processing steps.
  • Successfully patterned biomolecules without denaturation using the developed photoresist.

Conclusions:

  • The developed pH-responsive photoresist offers a novel approach for selective and mild surface immobilization of biomolecules.
  • This technology has significant potential for advancing biosensor development and biotechnological applications requiring precise biomolecular patterning.