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Updated: May 7, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
Published on: March 29, 2019
Inducible control of gene expression with destabilized Cre
Richard Sando1, Karsten Baumgaertel, Simon Pieraut
11] Department of Molecular and Cellular Neuroscience, The Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, California, USA. [2] The Kellogg School of Science and Technology, The Scripps Research Institute, La Jolla, California, USA.
Abstract:
Acute manipulation of gene and protein function in the brain is essential for understanding the mechanisms of nervous system development, plasticity and information processing. Here we describe a technique based on a destabilized Cre recombinase (DD-Cre) whose activity is controlled by the antibiotic trimethoprim (TMP). We show that DD-Cre triggers rapid TMP-dependent recombination of loxP-flanked ('floxed') alleles in mouse neurons in vivo and validate the use of this system for neurobehavioral research.
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