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Characterization of a layered methylene blue/vanadium oxide nanocomposite and its application in a reagentless H2O2
Xiaobo Zhang1, Dan Li, Fujun Yin
1Department of Chemical Engineering, Huaihai Institute of Technology, Lianyungang, 222005, People's Republic of China.
Abstract:
Layered nanocomposite of methylene blue (MB)-intercalated vanadium oxide was obtained through a simple hydrothermal synthesis method using MB, V2O5, and NaI as starting materials. The intercalation reaction was proven to be successful using X-ray diffraction pattern. The MB-V2O5 nanocomposite was characterized using a scanning electron micrograph, infrared spectra, thermogravimetric analysis, UV spectra, and electrochemical measurements. The intercalated MB cations showed a fine diffusion-controlled electrochemical redox process and facilitated the immobilized horseradish peroxidase's (HRP) good catalytic reduction upon H2O2. The as-prepared MB-V2O5/HRP biosensor showed a linear response to H2O2 over a range from 2.0 × 10(-6) to 9.5 × 10(-5) M with a detection limit of 9.7 × 10(-7) M (S/N ratio = 3).
