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LONG-TERM MONITORING OF THE PHYSICOCHEMICAL PROPERTIES OF SILICA-BASED NANOPARTICLES ON THE RATE OF ENDOCYTOSIS AND
Shin-Woo Ha1, Corinne E Camalier, M Neale Weitzmann
1Department of Chemistry, Seoul National University, Seoul Korea 151-74.
Soft Materials
|September 24, 2013
Summary
Silica nanoparticles enter osteoblasts, with surface charge affecting uptake speed but not toxicity. These nanoparticles are safely transferred to daughter cells during cell division, persisting for weeks.
Area of Science:
- Biomaterials Science
- Cell Biology
- Nanotechnology
Background:
- Nanomaterial properties like size, shape, and charge significantly influence their behavior in biological systems.
- Understanding nanoparticle-cell interactions is crucial for developing safe and effective nanomaterial-based therapies.
Purpose of the Study:
- To investigate the impact of surface charge on silica nanoparticle dynamics within murine pre-osteoblast cells (MC3T3-E1).
- To assess nanoparticle uptake kinetics, cell viability, cell division, exocytosis, and extracellular matrix interaction.
Main Methods:
- Utilized silica-based fluorescent nanoparticles with three surface modifications: positively charged (PTMA), negatively charged (OH), and neutrally charged (PEG).
- Analyzed nanoparticle interactions with MC3T3-E1 cells over time, monitoring endocytosis, cell viability, and cell division.
- Quantified nanoparticle transfer to daughter cells and assessed exocytosis and collagen matrix interactions.
Main Results:
- Positively charged (PTMA) nanoparticles showed the most rapid cellular uptake (within 2 hours).
- PEG and negatively charged (OH) nanoparticles exhibited slower uptake kinetics.
- Cell viability remained high (>80%) across all tested nanoparticle surface charges, indicating low toxicity.
- Nanoparticles were successfully transferred to daughter cells during mitosis, with decreased fluorescent intensity observed.
- Nanoparticles persisted intracellularly or within the collagen matrix for weeks with minimal exocytosis.
Conclusions:
- Silica nanoparticles, regardless of surface charge, can internalize into osteoblasts, though with varying kinetics.
- These nanoparticles demonstrate long-term intracellular persistence and are transferable to daughter cells without inducing significant toxicity.
- The findings support the potential of silica nanoparticles for applications in bone tissue engineering and regenerative medicine.
