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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: May 7, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

Methods to analyse composition and dynamics of macromolecular complexes.

Heinrich Heide1, Ilka Wittig

  • 1*Functional Proteomics, SFB815 Core Unit, Faculty of Medicine, Goethe-University, Theodor-Stern-Kai 7, D-60590 Frankfurt am Main, Germany.

Biochemical Society Transactions
|September 25, 2013
PubMed
Summary

Researchers explore proteomic methods for identifying macromolecular complexes, crucial for understanding cellular functions and diseases. These techniques aid in analyzing protein interactions and the complete complexome in biological samples.

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Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
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Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

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Last Updated: May 7, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
07:33

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • Macromolecular complexes are fundamental to numerous cellular processes, including biosynthesis, metabolism, DNA replication, and signal transduction.
  • Studying these complexes in health and disease offers critical insights into cellular physiology and pathophysiology.
  • Recent decades have seen significant advancements in identifying and understanding the dynamics of macromolecular complexes.

Purpose of the Study:

  • To provide an overview of proteomic methods for identifying protein-protein interactions.
  • To highlight recent developments in studying the entire complexome of biological samples.
  • To underscore the importance of analyzing macromolecular complexes for biological and medical research.

Main Methods:

  • Overview of established and emerging proteomic techniques for protein complex identification.
  • Focus on methods enabling the isolation of native protein complexes from cellular and tissue samples.
  • Discussion of microscopic and proteomic analyses for characterizing isolated complexes.

Main Results:

  • Proteomic methods offer powerful tools for dissecting macromolecular complexes.
  • Recent advancements facilitate the comprehensive study of the complexome.
  • These techniques are essential for characterizing protein-protein interactions within cellular systems.

Conclusions:

  • Proteomic analysis is vital for understanding the roles of macromolecular complexes in cellular functions.
  • The study of protein complexes provides insights into disease mechanisms.
  • Continued development of proteomic methods will enhance our ability to analyze the complexome.