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Updated: May 7, 2026

Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer
Published on: October 15, 2015
Translational dynamics of water at the phospholipid interface
Ken G Victor1, Jean-Pierre Korb, Robert G Bryant
1Chemistry Department, University of Virginia Charlottesville, Virginia, United States.
Abstract:
The residual water-proton magnetic relaxation dispersion profile obtained from suspensions of phospholipid vesicles in deuterium oxide was found to be a logarithmic function of the proton Larmor frequency at high magnetic field strengths, and independent of Larmor frequency at low magnetic field strengths. The residual proton relaxation is caused by dipole-dipole coupling between the residual water proton in otherwise deuterated water and the phospholipid protons. The logarithmic dependence on magnetic field strength is the signature of water-proton diffusive exploration on the interface that is approximately two-dimensionally constrained. Application of relaxation theory for two-dimensional diffusion to the spin-lattice relaxation data yields a translational correlation time of approximately 70 ps for water diffusing in the interface of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) vesicles.
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