Related Experiment Video
Updated: May 7, 2026
10:27
Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
Published on: July 14, 2015
Large volume monolithic stationary phases: preparation, properties, and applications
Acta Chimica Slovenica
|September 25, 2013
Summary
Monolithic chromatography supports offer superior flow-independent separations for large molecules like proteins and DNA. This article details the preparation and applications of large-volume monolithic columns for efficient purification.
Area of Science:
- Chromatography
- Bioseparations
- Materials Science
Background:
- Monoliths are the 4th generation of chromatographic supports, featuring a single piece of highly porous material.
- Interconnected flow-through pores enable convective transport, leading to flow-independent separations.
- This is crucial for purifying large molecules such as proteins, DNA, and viruses.
Purpose of the Study:
- To describe the preparation of large-volume monolithic columns.
- To highlight the main applications of these advanced chromatographic supports.
Main Methods:
- Preparation of large volume monolithic columns.
- Characterization of flow properties and separation capabilities.
Main Results:
- Monolithic columns provide flow-independent separations and high dynamic binding capacity.
- Effective purification of large molecular weight molecules is achieved.
Conclusions:
- Large volume monolithic columns are essential for efficient purification of biomolecules.
- Monolithic chromatography represents a significant advancement in separation science.
Related Concept Videos
01:17Gas Chromatography: Types of Columns and Stationary Phases
Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
01:11High-Performance Liquid Chromatography: Introduction
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
01:10Silica Gel Column Chromatography: Overview
Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
01:15Optimizing Chromatographic Separations
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
01:09Analyte Adsorption and Distribution
In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and solvents...
01:09Ion-Exchange Chromatography
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...