Cathodic adsorptive stripping voltammetric determination of prednisolone in pharmaceutical preparation and human urine

Differential pulse cathodic adsorptive stripping voltammetric method was developed for the determination of prednisolone based on electrochemical reduction of the drug at a hanging mercury drop electrode (HMDE) in 0.04 M Britton-Robinson buffer pH 3.5. The peak current varied linearly over the concentration range 7.21-144.2 ng/ml prednisolone with limit of detection and limit of quantification of 3.95 and 13.17 ng/ml prednisolone, respectively. The proposed method was successfully applied for the determination of the drug in commercial tablets and spiked human urine samples.