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Updated: May 7, 2026

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Published on: May 12, 2020
Preparation and Reactivity of 5-benzylidenebarbituric and 5-benzylidene-2-thiobarbituric Acids
Abstract:
The reaction between barbituric acid or 2-thiobarbituric acid and different benzaldehydes, dependent upon the applied reaction conditions, selectively yields three different product types, i.e. 5-benzylidene(thio)barbituric acids II, symetric Michael adducts III or 5-phenyl-1H-pyrano[2,3-d]pyrimidine-2,4(3H,5H)-diones and 5-phenyl-2-thioxo-2,3-dihydro-1H-pyrano[2,3-d]pyrimidin-4(5H)-ones IV. The reaction mechanisms and reactivity of different benzaldehydes are discussed.
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