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Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Crossing Over01:30

Crossing Over

Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I, duplicated...
Crossing Over01:34

Crossing Over

Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process called synapsis.
In order to...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

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Related Experiment Video

Updated: May 7, 2026

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

An alignment-free test for recombination.

Bernhard Haubold1, Linda Krause, Thomas Horn

  • 1Department of Evolutionary Genetics, Max-Planck-Institute for Evolutionary Biology, 24306 Plön, Institute for Neuro- and Bioinformatics, Lübeck University, 23562 Lübeck and Mathematical Stochastics, Mathematical Institute, Freiburg University, 79104 Freiburg, Germany.

Bioinformatics (Oxford, England)
|September 26, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces an efficient alignment-free method to detect genetic recombination by analyzing DNA sequence data. The new approach is fast enough for whole bacterial genomes and accurately identifies recombination events.

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Related Experiment Videos

Last Updated: May 7, 2026

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

Recombineering Homologous Recombination Constructs in Drosophila
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Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
11:40

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Published on: June 25, 2013

Area of Science:

  • Genomics
  • Computational Biology
  • Evolutionary Biology

Background:

  • Quantifying genetic recombination is crucial for understanding biological evolution.
  • Existing methods often rely on DNA sequence alignment, which can be computationally intensive.
  • There is a need for faster, alignment-free methods for recombination detection.

Purpose of the Study:

  • To develop an efficient, alignment-free method for detecting genetic recombination.
  • To enable rapid analysis of recombination across large genomic datasets, such as whole bacterial genomes.

Main Methods:

  • Utilizing the distribution of match lengths between DNA sequences.
  • Employing enhanced suffix arrays for efficient lookup of match length distributions.
  • Developing the 'rush' command-line program for method implementation.

Main Results:

  • The alignment-free method demonstrates comparable power to established tests for long sequences.
  • The method is sufficiently fast for whole bacterial genome analysis.
  • Successfully identified a strong recombination signal in *Escherichia coli* linked to a known horizontal gene transfer event.

Conclusions:

  • The proposed alignment-free method offers an efficient alternative for detecting genetic recombination.
  • This approach facilitates large-scale genomic studies of recombination.
  • The 'rush' program provides a practical tool for researchers to apply this method.