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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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Related Experiment Video

Updated: May 7, 2026

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
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Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

Capturing drug responses by quantitative promoter activity profiling.

K Kajiyama1, M Okada-Hatakeyama, Y Hayashizaki

  • 11] RIKEN Center for Life Science Technologies, Division of Genomic Technologies, Tsurumi-ku, Yokohama, Japan [2] Graduate School of Nanobioscience, Yokohama City University, Tsurumi-ku, Yokohama, Kanagawa, Japan.

CPT: Pharmacometrics & Systems Pharmacology
|September 27, 2013
PubMed
Summary

This study introduces a quantitative method for profiling cellular responses to drugs, improving upon microarray limitations. The new technique accurately measures promoter activity, offering deeper insights into drug effects on signaling pathways.

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Last Updated: May 7, 2026

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Area of Science:

  • Pharmacology
  • Genomics
  • Systems Biology

Background:

  • Quantitative analysis of cellular drug responses is crucial in pharmaceutical research.
  • Microarray technology has limitations in RNA coverage, sensitivity, and quantitativeness for monitoring cellular responses.
  • Accurate monitoring of cellular responses requires sensitive and quantitative methods.

Purpose of the Study:

  • To develop and apply a genome-wide, quantitative profiling method for cellular responses to drugs.
  • To monitor promoter activities using Cap Analysis of Gene Expression (CAGE) with a single-molecule sequencer.
  • To identify promoters affected by subtle inhibition of key signal-transduction pathways.

Main Methods:

  • Utilized Cap Analysis of Gene Expression (CAGE) with single-molecule sequencing.
  • Monitored promoter activities in MCF-7 cells.
  • Analyzed responses to inhibition of Ras-ERK and phosphatidylinositol-3-kinase-Akt signaling pathways.

Main Results:

  • Identified a distinct set of promoters responsive to subtle inhibition of Ras-ERK and Akt pathways.
  • Quantitatively explained promoter responses to epidermal growth factor receptor kinase inhibition.
  • Demonstrated the utility of highly quantitative promoter activity profiling.

Conclusions:

  • Highly quantitative promoter activity profiling offers unexplored utility in drug research.
  • This method provides a more accurate way to monitor cellular responses to drugs.
  • The findings advance the understanding of drug mechanisms and signaling pathways.