Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Commentary: Cancer-testis antigen lactate dehydrogenase C4 as a novel biomarker of male infertility and cancer.

Frontiers in oncology·2023
Same author

Incidence and risk predictors analysis of adverse donor reactions in whole blood donation.

Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine·2020
Same author

Deep venous thrombosis manifestation of common femoral vein leiomyoma.

Annals of the Royal College of Surgeons of England·2020
Same author

Establishing scald prevention measures in UK maternity units from takeaway drinks.

Burns : journal of the International Society for Burn Injuries·2020
Same author

Temperature dissociation of liquids in reusable thermoplastic containers-An eco-friendly scald risk?

Burns : journal of the International Society for Burn Injuries·2019
Same author

Acquired diaphragmatic hernia in a preterm low birth weight neonate.

Tropical doctor·2019
Same journal

Whole-body mass spectrometry imaging reveals metabolome and lipid peroxidation heterogeneity in zebrafish xenografts of esophageal squamous cell carcinoma.

Analytical and bioanalytical chemistry·2026
Same journal

A robust and validated method for the determination of 21 urinary metabolites of 15 plasticizers, including phthalates, DEHTP, and DINCH, by online SPE and liquid chromatography-tandem mass spectrometry.

Analytical and bioanalytical chemistry·2026
Same journal

A label-free membrane-based biosensor array with AuNP-modified PDMS for sensitive and specific detection of alpha-fetoprotein.

Analytical and bioanalytical chemistry·2026
Same journal

Smartphone-integrated one-step colorimetric glucose detection at physiological pH enabled by a haloperoxidase mimic.

Analytical and bioanalytical chemistry·2026
Same journal

Chemiluminescence functionalized magnetic nanoparticles-based biosensor for sensitive detection of glucose, uric acid, and cholesterol.

Analytical and bioanalytical chemistry·2026
Same journal

Single-cell mass spectrometry imaging: platform advances for multimodal spatial omics.

Analytical and bioanalytical chemistry·2026
See all related articles

Related Experiment Video

Updated: May 7, 2026

Microembossing: A Convenient Process for Fabricating Microchannels on Nanocellulose Paper-Based Microfluidics
03:58

Microembossing: A Convenient Process for Fabricating Microchannels on Nanocellulose Paper-Based Microfluidics

Published on: October 6, 2023

Developing new materials for paper-based diagnostics using electrospun nanofibers.

S J Reinholt1, A Sonnenfeldt, A Naik

  • 1Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY, USA.

Analytical and Bioanalytical Chemistry
|September 27, 2013
PubMed
Summary
This summary is machine-generated.

Electrospun nanofibers enhance paper-based lateral flow assays (LFAs). Functionalized poly(lactic acid) nanofibers improve antibody adsorption, enable quantification, and reduce nonspecific binding for sensitive bioassays.

More Related Videos

Vapor Phase Deposition of Electroactive Poly(3,4-ethylenedioxythiophene) onto Electrospun Commodity Polymer Nanofibers
08:28

Vapor Phase Deposition of Electroactive Poly(3,4-ethylenedioxythiophene) onto Electrospun Commodity Polymer Nanofibers

Published on: March 7, 2025

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
11:33

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

Related Experiment Videos

Last Updated: May 7, 2026

Microembossing: A Convenient Process for Fabricating Microchannels on Nanocellulose Paper-Based Microfluidics
03:58

Microembossing: A Convenient Process for Fabricating Microchannels on Nanocellulose Paper-Based Microfluidics

Published on: October 6, 2023

Vapor Phase Deposition of Electroactive Poly(3,4-ethylenedioxythiophene) onto Electrospun Commodity Polymer Nanofibers
08:28

Vapor Phase Deposition of Electroactive Poly(3,4-ethylenedioxythiophene) onto Electrospun Commodity Polymer Nanofibers

Published on: March 7, 2025

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
11:33

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

Area of Science:

  • Biomaterials Engineering
  • Analytical Chemistry
  • Biosensor Technology

Background:

  • Paper-based lateral flow assays (LFAs) are widely used for rapid diagnostics.
  • Improving the sensitivity and specificity of LFAs is crucial for advanced bioassays.
  • Electrospun nanofibers offer unique properties for functionalizing assay platforms.

Purpose of the Study:

  • To investigate the use of electrospun nanofibers as functional materials in paper-based LFAs.
  • To develop and optimize PLA-based nanofiber formulations for enhanced LFA performance.
  • To demonstrate key functionalities including antibody adsorption, result quantification, and reduction of nonspecific binding.

Main Methods:

  • Electrospinning of poly(lactic acid) (PLA) doped with functional polymers (PEG, K3-Brij76).
  • Assembly of LFAs utilizing capillary action through nanofiber mats.
  • Development of sandwich assays for detecting Escherichia coli O157:H7.
  • Utilizing antifouling block copolymers to minimize nonspecific binding.

Main Results:

  • PLA-based nanofibers enabled efficient LFA assembly, unlike PVA.
  • Functionalized nanofibers demonstrated effective antibody adsorption and capture zone formation.
  • A sensitive assay for E. coli O157:H7 achieved a detection limit of 1.9 × 10^4 cells.
  • Nonspecific binding was successfully eliminated using antifouling block copolymers.

Conclusions:

  • Electrospun nanofibers significantly enhance the capabilities of paper-based LFAs.
  • Functionalized nanofibers provide a versatile platform for developing sensitive and specific bioassays.
  • This approach offers advantages over traditional paper-based diagnostic devices.