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NiO Nanoflowers for Non-Enzymatic Amperometric Detection of Glucose
Published on: December 30, 2025
Wide linear-range detecting nonenzymatic glucose biosensor based on CuO nanoparticles inkjet-printed on electrodes
Rafiq Ahmad1, Mohammad Vaseem, Nirmalya Tripathy
1Department of BIN Fusion Technology, School of Semiconductor and Chemical Engineering, Chonbuk National University , 567 Baekje-daero, Deokjin-gu, Jeonju 561-756, Republic of Korea.
Analytical Chemistry
|September 28, 2013
Summary
Inkjet-printed copper oxide nanoparticles create a sensitive, stable, and reproducible nonenzymatic glucose biosensor. This novel sensor accurately detects glucose in human serum, showing minimal interference from common species.
Area of Science:
- Electrochemistry
- Nanomaterials Science
- Biosensor Technology
Background:
- Nonenzymatic glucose biosensors offer an alternative to enzyme-based systems.
- Copper oxide nanoparticles (CuO NPs) show promise for electrochemical sensing applications.
- Inkjet printing provides a scalable method for fabricating electrode materials.
Purpose of the Study:
- To develop and characterize a nonenzymatic glucose biosensor using inkjet-printed CuO NPs on silver electrodes.
- To evaluate the sensor's sensitivity, detection range, stability, and selectivity.
- To assess the sensor's applicability for glucose determination in real biological samples like human serum.
Main Methods:
- Fabrication of CuO NPs/Ag electrodes via inkjet printing.
- Electrochemical characterization using cyclic voltammetry and amperometry.
- Evaluation of sensor performance including sensitivity, linear range, and detection limit.
- Assessment of interference from common biological species and sugar derivatives.
- Validation using human serum samples.
Main Results:
- High sensitivity (2762.5 μA mM⁻¹ cm⁻²) achieved at +0.60 V.
- Wide linear detection range from 0.05 to 18.45 mM.
- Low detection limit of approximately 0.5 μM (S/N = 3).
- Excellent long-term stability and reproducibility due to CuO NP stability and pore-like Ag surface structure.
- Negligible interference from ascorbic acid (AA), uric acid (UA), dopamine (DA), and insignificant interference from sugar derivatives.
Conclusions:
- Inkjet-printed CuO NPs on Ag electrodes form a highly effective nonenzymatic glucose biosensor.
- The sensor demonstrates superior performance characteristics, including sensitivity, stability, and selectivity.
- The developed biosensor is suitable for accurate glucose concentration analysis in human serum samples.
