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Related Concept Videos

Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
Exocytosis00:51

Exocytosis

Exocytosis is used to release material from cells. Like other bulk transport mechanisms, exocytosis requires energy.
Exocytosis00:50

Exocytosis

Exocytosis is a process that releases molecules outside the cell. Like other bulk transport mechanisms, exocytosis requires energy.
Exocytosis is the opposite of endocytosis, which brings molecules inside the cell. Sometimes, the released materials are signaling molecules. For example, neurons typically use exocytosis to release neurotransmitters. Cells also use exocytosis to insert proteins such as ion channels into their cell membranes, secrete proteins for use in the extracellular matrix, or...
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...

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Related Experiment Video

Updated: May 7, 2026

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells
10:21

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

Published on: September 16, 2020

Membrane traffic: the exocyst meets the cell cycle.

Patrick Brennwald1

  • 1Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, 522 Taylor Hall, CB 7090, Chapel Hill, NC 27599-7090, USA.

Current Biology : CB
|September 28, 2013
PubMed
Summary

Researchers discovered a new way cell transport is blocked during cell division. This involves a specific enzyme complex directly phosphorylating a key protein, halting vesicle movement from the Golgi apparatus.

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Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Exocytic transport is crucial for cell function.
  • Regulation of vesicle transport during mitosis is not fully understood.
  • The exocyst complex plays a role in tethering vesicles to the plasma membrane.

Purpose of the Study:

  • To identify novel regulatory mechanisms controlling exocytic transport during mitosis.
  • To elucidate the molecular players involved in inhibiting Golgi-derived vesicle transport in dividing cells.

Main Methods:

  • Investigated the regulation of exocytic transport during mitosis.
  • Utilized biochemical assays to study protein phosphorylation.
  • Examined the interaction between cyclin-dependent kinase complexes and exocyst subunits.

Main Results:

  • Identified a novel regulatory event inhibiting exocytic transport of specific Golgi-derived vesicles during mitosis.
  • Demonstrated direct phosphorylation of an exocyst subunit by a cyclin-dependent kinase complex.
  • Showcased a mechanism that halts vesicle movement during cell division.

Conclusions:

  • A specific cyclin-dependent kinase complex directly phosphorylates an exocyst subunit.
  • This phosphorylation event inhibits exocytic transport of certain Golgi-derived vesicles during mitosis.
  • Provides new insights into the cell cycle-dependent regulation of membrane trafficking.