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Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology
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CDOM fluorescence as a proxy of DOC concentration in natural waters: a comparison of four contrasting tropical

E Rochelle-Newall1, F D Hulot, J L Janeau

  • 1IRD-Bioemco, UMR 7618, c/o Soils and Fertilisers Research Institute (SFRI), Dong Ngac, Tu Liem District, Hanoi, Vietnam, emma.rochelle-newall@ird.fr.

Environmental Monitoring and Assessment
|September 28, 2013
PubMed

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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.