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A biotyping scheme for Salmonella livingstone
M O Odongo1, I M McLaren, J E Smith
1Veterinary Research Laboratory, PO Kabete, Kenya.
The British Veterinary Journal
|January 1, 1990
Summary
Salmonella livingstone, a common UK pathogen, was characterized using biochemical tests and plasmid profiles. This combined approach allows for effective
Area of Science:
- Microbiology
- Epidemiology
Background:
- Salmonella livingstone is a frequently isolated serotype in the United Kingdom.
- Characterization of bacterial isolates is crucial for understanding transmission dynamics.
Purpose of the Study:
- To characterize 70 isolates of Salmonella livingstone.
- To develop a method for 'fingerprinting' Salmonella livingstone for epidemiological studies.
Main Methods:
- Biochemical tests were used to assess growth in d-tartrate, l-tartrate, and Stern's glycerol.
- Plasmid profile analysis was employed to identify light (<9.5 MD) and heavy (>30 MD) plasmids.
- Combined biotyping and plasmid profiling were used for isolate characterization.
Main Results:
- Salmonella livingstone isolates were divided into four groups based on biochemical properties.
- Plasmid profile analysis further subdivided isolates, with 24 possessing light plasmids and four possessing heavy plasmids.
- The combination of biotyping and plasmid profiling provided a detailed characterization of the isolates.
Conclusions:
- Biotyping and plasmid profile analysis are effective methods for characterizing Salmonella livingstone.
- This combined approach can serve as a basis for a 'fingerprinting' scheme for epidemiological surveillance.
- Understanding the genetic diversity of Salmonella livingstone aids in tracking its spread.