Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cobalamin-dependent methionine synthase.

R V Banerjee1, R G Matthews

  • 1Biophysics Research Division, University of Michigan, Ann Arbor 48109.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|March 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Safety of ultrasonics in endodontics.

General dentistry·2012
Same author

Movies, metals, bonds and barriers.

Current opinion in structural biology·2001
Same author

Effects of common polymorphisms on the properties of recombinant human methylenetetrahydrofolate reductase.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same author

Cobalamin-dependent methyltransferases.

Accounts of chemical research·2001
Same author

Mapping the interactions between flavodoxin and its physiological partners flavodoxin reductase and cobalamin-dependent methionine synthase.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same author

Activation from a distance: roles of Lrp and integration host factor in transcriptional activation of gltBDF.

Journal of bacteriology·2001
Same journal

Sinomenine Regulates the TRIM32/IRF1/TRAF6 Axis to Inhibit Pyroptosis in Atopic Dermatitis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

Urolithin A Mitigates Renal Fibrosis by Promoting Fatty Acid Oxidation Through Orchestrating β-Catenin Signaling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

MiR-124 Inhibits Lipid Deposition in Mouse Liver by Targeting the Trib3/Hnf4α Pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

TCN2 Drives Psoriasis-Like Inflammation and Keratinocyte Hyperproliferation, Correlating With IL-1β and STAT3 Activation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

The Synthetic Melanocortin Agonist NDP-MSH Ameliorates THSD7A-Associated Membranous Nephropathy in an Active Immunization Mouse Model.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

Brain-Derived Neurotrophic Factor and Associated Signaling in Kidney Diseases.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
See all related articles

Cobalamin-dependent methionine synthase is crucial for folate metabolism. Its inhibition causes anemia and neurological issues, presenting a potential target for cancer therapy.

Area of Science:

  • Biochemistry
  • Enzymology
  • Cellular Metabolism

Background:

  • Cobalamin-dependent methionine synthase (MS) is vital for methyl group transfer from N5-methyltetrahydrofolate to homocysteine.
  • Enzyme inhibition by cobalamin deficiency or nitrous oxide impairs MS activity.
  • Severe MS inhibition in humans leads to megaloblastic anemia and spinal cord degeneration.

Purpose of the Study:

  • To review recent advancements in understanding the catalysis and regulation of methionine synthase.
  • To explore the enzyme's role in folate metabolism and its potential as a chemotherapeutic target.

Main Methods:

  • Literature review of bacterial and mammalian methionine synthase.
  • Analysis of enzyme inhibition mechanisms and consequences.
  • Discussion of mechanistic insights for rational inhibitor design.

Related Experiment Videos

Main Results:

  • MS activity is essential for maintaining folate pools for nucleotide biosynthesis.
  • Inhibition of mammalian MS disrupts folate incorporation into cells.
  • Understanding enzyme mechanisms is key to developing targeted inhibitors.

Conclusions:

  • Methionine synthase is a critical enzyme in folate and one-carbon metabolism.
  • Its inhibition has significant pathological consequences, including anemia and neurological damage.
  • Targeting methionine synthase offers a promising strategy for cancer chemotherapy.