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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Updated: May 7, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Alternative spliceosome assembly pathways revealed by single-molecule fluorescence microscopy.

Inna Shcherbakova1, Aaron A Hoskins, Larry J Friedman

  • 1Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA; Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA.

Cell Reports
|October 1, 2013
PubMed
Summary

Spliceosome assembly in yeast can occur through two distinct pathways: U1-first or U2-first. Both pathways lead to functional spliceosomes, indicating flexibility in gene expression. This finding impacts our understanding of spliceosome assembly mechanisms.

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Last Updated: May 7, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy
05:58

In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy

Published on: September 6, 2024

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Spliceosome-mediated intron removal is crucial for eukaryotic gene expression.
  • Previous models proposed a strictly ordered, U1-snRNP-first pathway for yeast spliceosome assembly.

Purpose of the Study:

  • To investigate the initial steps of spliceosome assembly in yeast.
  • To determine if alternative assembly pathways exist beyond the U1-first model.

Main Methods:

  • Colocalization single-molecule spectroscopy was employed.
  • Initial spliceosome assembly was monitored on eight different Saccharomyces cerevisiae pre-mRNAs.

Main Results:

  • Active yeast spliceosomes can assemble via both U1-first and U2-first pathways.
  • Both pathways produce functionally equivalent prespliceosomes.
  • Both pathways are operational on all studied introns, with varying flux.

Conclusions:

  • Multiple pathways exist for functional spliceosome assembly in yeast.
  • These findings challenge the exclusively ordered model and offer insights into spliceosome assembly coordination.