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Updated: May 7, 2026
Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
Published on: August 3, 2018
Co-regulated expression of TGF-β Variants and miR-21 in bladder cancer
Hamideh Monfared1, Seyed Amir Mohsen Ziaee, Mahmoud Hashemitabar
1Molecular Genetics Department, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran. hamideh_monfared@yahoo.com.
Purpose:
To investigate a potential alteration in the expression levels of transforming growth factor β (TGF-β) and miR-21 in bladder cancer tissues.
Material And Methods:
Using real-time polymerase chain reaction (PCR) method, we examined a potential correlated expression of miR-21 and TGF-β variants in 30 bladder tumors and their marginal/non-tumor biopsy specimens obtained from the same patients.
Results:
Our data revealed a significant down-regulation of TGF-β variants (P = .03) along with a non-significant alteration in the expression of miR-21 in tumor vs. non-tumor samples. However, in contrast to low-grade tumors, the expression of miR-21 was upregulated in high-grade ones, and the expression level can efficiently discriminate low-grade tumors from high-grade ones (P = .03).
Conclusion:
In accordance to the observed similarity between TGF-β variants and miR-21 gene expression alterations in bladder tumors, treating 5637 bladder cancer cell line with TGF-β recombinant protein caused a significant upregulation of miR-21. The later finding further confirmed a correlated expression of TGF-β and miR-21 in bladder tumors.
