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Published on: October 20, 2019
[Analysis of allele dropout at TH01 locus in paternity testing]
Li Lai1, Xiao-li Shen, Shi-jie Xue
1Forensic Science Institute of Fujian Provincial Hospital, Fujian Provincial Key Laboratory of Cardiovascular Disease, Provincial Clinical College of Fujian Medical University, Fuzhou, Fujian 350001, P. R. China.
Objective:
To analyze allele dropout at TH01 locus in paternity testing in order to determine the accurate genotype.
Methods:
To use a two STR loci genotyping system to verify an abnormal genotype for the TH01 locus with PCR using specific primers, cloning and DNA sequencing.
Results:
A rare allele at TH01 locus named 5.2, which was undetectable with PowerPlex 21 system, was detected with an Identifiler system.
Conclusion:
Genetic variations may result in rare alleles and loci loss. To avoid misjudgment, laboratories should have a variety of methods for detecting loci loss.
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