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Effect of Sophora subprosrate polysaccharide on oxidative stress induced by PCV2 infection in RAW264.7 cells
Zi-Jie Su1, Ying-Yi Wei, Dan Yin
1College of Animal Science and Technology, Guangxi University, Nanning 530005, PR China.
Abstract:
In this study, an oxidative stress model was first developed in a mouse macrophage cell line (RAW264.7 cells) by infecting the cells with porcine circovirus type 2 (PCV2). The regulatory effect of Sophora subprosrate polysaccharide (SSP) on PCV2-induced oxidative stress was investigated. The results showed that after infection with PCV2, reactive oxygen species (ROS) and nitric oxide (NO) production, myeloperoxidase (MPO) activity, and inducible nitric oxide synthase (iNOS) expression were significantly increased. Meanwhile, the ratio of reduced glutathione to oxidized glutathione (GSH/GSSG) and hydroxyl radical prevention capacity were greatly reduced. These data indicate successful creation of an oxidative stress model in RAW264.7 cells. A dramatic decrease in cell viability was observed in the cells exposed to oxidative stress compared to the control. When the cells were treated with SSP in concentrations of 100, 200 or 400 μg/mL post PCV2 infection, an increase in the GSH/GSSG ratio and hydroxyl radical prevention capacity was observed. We also observed decreased ROS and NO production, MPO activity, and iNOS expression in the infected cells. Our results demonstrated that PCV2 infection was able to induce oxidative stress in RAW264.7 cells and that SSP could reduce the negative effects resulting from the PCV2 infection.
Insights
Porcine circovirus type 2 (PCV2) infection induced oxidative stress in mouse macrophages. Sophora subprosrate polysaccharide (SSP) treatment mitigated these negative effects, restoring cellular health and reducing oxidative damage markers.
Area of Science:
- Immunology
- Cell Biology
- Pharmacology
Background:
- Porcine circovirus type 2 (PCV2) infection is a significant concern in swine health.
- PCV2 infection can lead to cellular damage through oxidative stress.
- Understanding the mechanisms of PCV2-induced oxidative stress is crucial for developing effective treatments.
Purpose of the Study:
- To establish an oxidative stress model using PCV2-infected mouse macrophage (RAW264.7) cells.
- To investigate the regulatory effect of Sophora subprosrate polysaccharide (SSP) on PCV2-induced oxidative stress.
- To evaluate SSP's potential to counteract PCV2-induced cellular damage.
Main Methods:
- Development of an oxidative stress model in RAW264.7 cells via PCV2 infection.
- Assessment of oxidative stress markers including reactive oxygen species (ROS), nitric oxide (NO), myeloperoxidase (MPO) activity, and inducible nitric oxide synthase (iNOS) expression.
- Measurement of the glutathione redox ratio (GSH/GSSG) and hydroxyl radical scavenging capacity.
- Treatment of infected cells with varying concentrations of SSP (100, 200, 400 μg/mL) to assess its protective effects.
Main Results:
- PCV2 infection successfully induced oxidative stress in RAW264.7 cells, evidenced by increased ROS, NO, MPO activity, and iNOS expression.
- PCV2 infection significantly reduced the GSH/GSSG ratio and hydroxyl radical scavenging capacity, leading to decreased cell viability.
- SSP treatment dose-dependently reversed these effects, increasing the GSH/GSSG ratio and hydroxyl radical scavenging capacity while decreasing ROS, NO, MPO, and iNOS levels.
Conclusions:
- PCV2 infection is capable of inducing significant oxidative stress in macrophages.
- Sophora subprosrate polysaccharide (SSP) exhibits potent antioxidant and protective properties against PCV2-induced cellular damage.
- SSP demonstrates potential as a therapeutic agent for mitigating PCV2-related pathologies.