Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Label-Free Holographic Imaging Flow Cytometry With Deep-Learning-Based Detection and Classification of Thousands of Cells Per Second.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026

Same author

Label-free refractive index mapping of human sperm cells.

Biomedical optics express·2025

Same author

Rare cell classification using label-free imaging flow cytometry <i>via</i> motion-sensitive-triggered interferometry.

Lab on a chip·2025

Same author

Enhancing frozen histological section images using permanent-section-guided deep learning with nuclei attention.

Scientific reports·2025

Same author

Resolution-enhanced quantitative phase imaging of blood platelets using a generative adversarial network.

Journal of the Optical Society of America. A, Optics, image science, and vision·2025

Same author

The Synergic Effect of Tubal Endometriosis and Women's Aging on Fallopian Tube Function: Insights from a 3D Mechanical Model.

Bioengineering (Basel, Switzerland)·2024

Search research articles

Related Experiment Video

Updated: May 7, 2026

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

Published on: June 5, 2019

Wide-field interferometric phase microscopy with molecular specificity using plasmonic nanoparticles.

Nir A Turko, Anna Peled, Natan T Shaked

Journal of Biomedical Optics

|October 2, 2013

Summary

We developed a new method using gold nanoparticles (AuNPs) and interferometric phase microscopy (IPM) to visualize specific molecules within cells. This technique enables wide-field, subcellular imaging without scanning, offering a novel approach for biological research.

More Related Videos

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

Published on: September 27, 2011

Related Experiment Videos

Last Updated: May 7, 2026

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

Published on: June 5, 2019

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

Published on: September 27, 2011

Area of Science:

Biophotonics
Nanotechnology
Cellular Imaging

Background:

Interferometric phase microscopy (IPM) offers label-free imaging but lacks molecular specificity.
Gold nanoparticles (AuNPs) exhibit plasmonic resonance, enabling photothermal (PT) effects upon light excitation.

Purpose of the Study:

To introduce a method for enhancing molecular specificity in wide-field IPM using AuNPs.
To demonstrate subcellular imaging of specific molecular targets within biological cells.

Main Methods:

Conjugating AuNPs to antibodies targeting specific cell surface receptors (e.g., EGFR).
Exciting AuNPs with time-modulated light at their absorption peak to induce a photothermal effect.
Utilizing wide-field IPM to detect phase shifts caused by local temperature changes around AuNPs.
Applying Fourier analysis to wide-field image sequences for AuNP localization and mapping.

Main Results:

Achieved wide-field imaging of photothermal phase signals from clusters of as few as 16 isolated AuNPs.
Successfully imaged AuNPs conjugated to EGFR antibodies in EGFR-overexpressing cancer cells.
Demonstrated subcellular resolution imaging without the need for total internal reflection or scanning.

Conclusions:

This study presents the first wide-field interferometric photothermal imaging at the subcellular level.
The developed method adds molecular specificity to IPM, enabling targeted visualization of biomarkers in cells.
This technique holds promise for advanced biological and biomedical imaging applications.