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Physiological effects of pH changes on colloid osmotic pressures
The American Journal of Physiology
|June 1, 1985
Summary
Physiological pH variations significantly impact colloid osmotic pressure (COP) in body fluids. Increased pH linearly elevates COP in plasma and proteins, influencing fluid distribution.
Area of Science:
- Biochemistry
- Physiology
- Fluid Dynamics
Background:
- Colloid osmotic pressure (COP) is crucial for fluid balance.
- Plasma proteins and interstitial substances contribute to COP.
- pH influences molecular charge and interactions.
Purpose of the Study:
- To investigate the impact of pH variations on body fluid COP.
- To understand how pH affects plasma and interstitial fluid pressure.
- To determine the physiological relevance of pH in fluid regulation.
Main Methods:
- Measured COP of human plasma, albumin, and Wharton's jelly across a pH range (6.0-8.0).
- Assessed pH sensitivity of different substances, including hyaluronate and Dextran 70.
- Developed a formula to describe COP changes with pH and concentration.
Main Results:
- COP increased linearly with pH for plasma, albumins, and hyaluronate.
- Plasma and albumins showed similar pH sensitivity.
- Hyaluronate exhibited significantly higher pH sensitivity than plasma.
- Dextran 70 showed no pH dependency.
- A formula was derived: COP = COPpH 7.4 [1.00 + 0.01C (pH -7.40)] for plasma and albumins.
Conclusions:
- Physiological pH fluctuations influence plasma COP and interstitial fluid pressure.
- These pH-mediated changes may play a key role in regulating transcapillary fluid exchange.
- Understanding pH effects on COP is vital for comprehending body fluid homeostasis.