Related Experiment Video
Updated: May 7, 2026

12:15
Calcium Imaging of Cortical Neurons using Fura-2 AM
Published on: January 19, 2009
Measurement of calibration constants for quantitative calcium fluorimetry
Cold Spring Harbor Protocols
|October 3, 2013
Abstract:
A careful calibration of the fluorescence setup is essential when using Fura-2 for quantitative fluorimetry. The calibration procedure described here is used for experiments in which the indicator dye is introduced into the cell through a patch-clamp pipette. The fluorescence ratio is determined in whole-cell recordings for three different calibration parameters that reflect different [Ca(2+)]: very high (R1), intermediate (Keff), and zero (R0).
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