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Related Concept Videos

Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
Ligand-gated Ion Channels01:19

Ligand-gated Ion Channels

Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that include the...
Ligand-gated Ion Channels01:19

Ligand-gated Ion Channels

Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
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Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that include the...
Mechanisms of Membrane-bending01:15

Mechanisms of Membrane-bending

The living membranes are flexible due to their fluid mosaic nature; however, their bending into different shapes is an active process regulated by specific lipids and proteins. The membrane bending can be transient as seen in vesicles or stable for a long time as in microvilli. Cells regulate the size, location, and duration of the membrane curvature.
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Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...

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Related Experiment Video

Updated: May 7, 2026

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein
09:59

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein

Published on: March 9, 2015

Modeling the conformational changes underlying channel opening in CFTR.

Kazi S Rahman1, Guiying Cui, Stephen C Harvey

  • 1Petit Institute of Bioengineering and Bioscience and School of Biology, Georgia Institute of Technology, Atlanta, Georgia, United States of America.

Plos One
|October 3, 2013
PubMed
Summary
This summary is machine-generated.

Cystic fibrosis transmembrane conductance regulator protein (CFTR) mutations cause CF. Molecular dynamics simulations reveal a conformational wave initiating ATP-dependent channel gating, crucial for CFTR function.

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Last Updated: May 7, 2026

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein
09:59

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein

Published on: March 9, 2015

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes
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Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
11:55

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling

Published on: May 29, 2011

Area of Science:

  • Biophysics
  • Molecular Biology
  • Genetics

Background:

  • Cystic fibrosis (CF) is caused by mutations in the CFTR gene.
  • Understanding CFTR protein conformational changes is key to CF pathogenesis.
  • Previous models lacked resolution on channel gating dynamics.

Purpose of the Study:

  • To elucidate the conformational transitions of CFTR during channel opening.
  • To investigate the mechanism of ATP-dependent gating in CFTR.

Main Methods:

  • Construction of closed- and open-state CFTR homology models.
  • Targeted molecular dynamics simulations of CFTR conformational changes.
  • Experimental validation of predicted structural changes.

Main Results:

  • Simulations identified a conformational wave driving channel opening.
  • This wave propagates from nucleotide-binding domains to the pore.
  • A novel, experimentally confirmed salt bridge disruption was observed.

Conclusions:

  • The study provides a mechanistic model for CFTR ATP-dependent gating.
  • These findings enhance understanding of CFTR ion channel function.
  • The research offers insights into CFTR dysfunction in cystic fibrosis.