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Related Experiment Video

Updated: May 7, 2026

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Published on: January 16, 2018

Field-portable pixel super-resolution colour microscope.

Alon Greenbaum¹, Najva Akbari, Alborz Feizi

¹Electrical Engineering Department, University of California Los Angeles, Los Angeles, California, United States of America.

|October 3, 2013

Summary

This summary is machine-generated.

We developed a portable, lensfree color microscope using digital holography for wide-field imaging. This compact device achieves sub-micron resolution and is ideal for remote medical diagnostics.

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Area of Science:

Optics and Photonics
Biomedical Imaging
Microscopy

Background:

Digital holographic microscopy offers label-free imaging capabilities.
Traditional holographic methods often suffer from color artifacts and limited field-of-view.
Field-portable microscopy is crucial for point-of-care diagnostics and remote sensing.

Purpose of the Study:

To develop a field-portable, lensfree color microscope with a wide field-of-view.
To overcome color artifacts in holographic imaging using advanced algorithms.
To demonstrate the capability for high-resolution imaging of biological samples.

Main Methods:

Partially-coherent digital in-line holography was employed.
A novel colorization algorithm merged with source-shifting multi-height pixel super-resolution was utilized.
Image processing involved transforming RGB to YUV color space for artifact mitigation.

Main Results:

A field-portable microscope (<145g, <17x6x5 cm) was successfully built.
Lensfree color images with a wide field-of-view (>20 mm²) were rendered.
Sub-micron spatial resolution was confirmed using a USAF test chart.
Successful imaging of Papanicolaou (Pap) smears demonstrated practical application.

Conclusions:

The developed computational holographic microscope is compact and suitable for field use.
The integrated image processing effectively reduces color artifacts, enabling high-quality color holographic imaging.
This technology holds potential for telemedicine in resource-limited settings.