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Packaged DNA. An elastic model.

G S Manning

    Cell Biophysics
    |March 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    DNA

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    Area of Science:

    • Biophysics
    • Molecular Biology
    • Polymer Physics

    Background:

    • DNA's elastic instability when straight, particularly under charge neutralization, influences its conformation.
    • Macroscopic rod theories offer partial insights into DNA's intrinsic bending capabilities.
    • Flexible kinking at open base pairs is a dominant DNA bending mechanism in solution.

    Purpose of the Study:

    • To deepen the theory of DNA elastic bending on a persistence length scale.
    • To explain DNA torus formation and its potential role in polynucleosome structure.
    • To quantitatively predict DNA bending radii based on molecular mechanisms.

    Main Methods:

    • Review and extension of elastic bending theories for DNA.
    • Application of quasi-Eulerian theory focusing on open base pair kinking.

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  • Comparison of theoretical predictions with experimental observations of DNA toruses and supercoils.

    • Main Results:

    • A stable bent conformation of DNA arises from elastic instability under charge neutralization.
    • The quasi-Eulerian theory quantitatively predicts the minimum radius of DNA toruses (approx. 16 nm).
    • Optimal stability for DNA toruses occurs at ~90% neutralization of phosphate groups by trivalent cations.

    Conclusions:

    • Open base pairs are crucial for DNA's equilibrium solution conformation and bending.
    • The predicted stable curvature of charge-neutralized DNA aligns with polynucleosome supercoil dimensions.
    • Further experimental validation is needed to confirm the link between DNA curvature and solenoid structure.