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The polymerization agent, 2-methyl-4'-(methylthio)-2-morpholinopropiophenone induces caspases-3/7 in human blood

Yoichi Kawasaki1, Kenta Yagi, Chiaki Tsuboi

  • 1Department of Pharmacy, Okayama University Hospital.

Biological & Pharmaceutical Bulletin
|October 4, 2013
PubMed

Insights

The photoinitiator 2-methyl-4'-(methylthio)-2-morpholinopropiophenone (MTMP) induces apoptosis, a programmed cell death, in human peripheral blood mononuclear cells (MNC). This occurs via a caspase-dependent pathway, confirmed by observing time-dependent changes in MNC viability and caspase activity.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Toxicology

Background:

  • The photoinitiator MTMP was previously detected in intravenous injection solutions.
  • MTMP exhibits cytotoxicity towards normal human peripheral blood mononuclear cells (MNC).
  • The specific mechanism of MTMP-induced cell death (apoptosis vs. necrosis) in MNC was unclear.

Purpose of the Study:

  • To investigate whether MTMP induces apoptosis or necrosis in normal human PB MNC.
  • To analyze the time-dependent effects of MTMP on MNC viability and cell death pathways.
  • To assess the role of caspases in MTMP-induced cell death.

Main Methods:

  • In vitro study using normal human peripheral blood (PB) mononuclear cells (MNC).
  • Exposure of MNC to MTMP to observe time-dependent changes in viability.
  • Assessment of caspase-3/7 activity as an indicator of apoptosis.

Main Results:

  • MTMP exposure led to time-dependent decreases in MNC viability.
  • MTMP was found to induce apoptosis in normal human PB MNC after 24 hours.
  • Caspase-3/7 activity increased in a time-dependent manner upon MTMP exposure.

Conclusions:

  • MTMP induces apoptosis in normal human peripheral blood mononuclear cells.
  • The observed apoptosis is mediated through a caspase-dependent pathway.
  • These findings highlight the potential cellular toxicity of MTMP in vitro.

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