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Updated: May 7, 2026

Immunofluorescent Staining for Visualization of Heterochromatin Associated Proteins in Drosophila Salivary Glands
Published on: August 21, 2021
Effete, an E2 ubiquitin-conjugating enzyme with multiple roles in Drosophila development and chromatin organization
Francesca Cipressa1, Giovanni Cenci1
1Dipartimento di Biologia e Biotecnologie "C. Darwin"; SAPIENZA Università di Roma; Roma, Italy.
Abstract:
The Drosophila effete gene encodes an extremely conserved class I E2 ubiquitin-conjugating enzyme. Growing evidence indicates that Eff is involved in many cellular processes including eye development, maintenance of female germline stem cells, and regulation of apoptosis. Eff is also a major component of Drosophila chromatin and it is particularly enriched in chromatin with repressive properties. In addition, Eff is required for telomere protection and to prevent telomere fusion. Consistent with its multiple roles in chromatin maintenance, Eff is also one of the rare factors that modulate both telomere-induced and heterochromatin-induced position effect variegation.
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