Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

The DNA Replication Fork

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...

The DNA Replication Fork

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...

The Replisome

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...

The Replisome

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Genome-wide screening identifies ZFP91 as a key regulator of EVI1 in myeloid leukemia.

Oncogene·2026

Same author

Parameterisation of epidemiological models from small field experiments: A case study of banana bunchy top virus transmission.

Mathematical biosciences·2026

Same author

Molecular tension indicators reveal unexpectedly complex regulation of tension in live mouse organs.

Communications biology·2026

Same author

Eukaryotic Replisome Components Cooperate to Process Histones During Chromosome Replication.

Cell reports·2025

Same author

Mass production of IgY-containing tablets for COVID-19 transmission control.

Scientific reports·2025

Same author

Developing a spatio-temporal model for banana bunchy top disease: leveraging remote sensing and survey data.

Frontiers in plant science·2025

Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026

Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026

Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026

Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026

Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026

Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 7, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

The dynamics of genome replication using deep sequencing.

Carolin A Müller¹, Michelle Hawkins, Renata Retkute

¹School of Life Sciences, The University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK, Deep Seq, The University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK, Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, 113-0032, Japan and Institute for Complex Systems and Mathematical Biology, The University of Aberdeen, Aberdeen, AB24 3UE UK.

Nucleic Acids Research

|October 4, 2013

Summary

This summary is machine-generated.

Researchers developed new deep sequencing methods to map DNA replication origins in yeast. These techniques precisely measure origin activity and genome replication timing, providing a valuable resource for eukaryotic genome studies.

More Related Videos

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

Related Experiment Videos

Last Updated: May 7, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

Area of Science:

Molecular Biology
Genomics
Cell Biology

Background:

Eukaryotic genome replication initiates from multiple DNA replication origins.
Understanding the precise location and activity of these origins is crucial for genome stability and replication dynamics.

Purpose of the Study:

To develop and apply novel deep sequencing approaches for precise measurement of DNA replication origin location and activity in Saccharomyces cerevisiae.
To generate high-resolution replication timing profiles and analyze replication dynamics in eukaryotic cells.

Main Methods:

Synchronous S-phase cultures to measure DNA copy number increase for precise genome replication determination.
Stalling replication forks near initiation sites to map origin locations via copy number enrichment.
Fluorescence-activated cell sorting (FACS) for generating replication timing profiles from asynchronous cultures.
Marker Frequency Analysis (MFA) applied to exponentially growing cultures for direct measurement of replication dynamics.

Main Results:

Precise determination of genome replication and mapping of DNA replication origins in Saccharomyces cerevisiae.
Demonstrated indistinguishable replication profiles between haploid and diploid cells, indicating shared origin usage.
Successfully applied marker frequency analysis (MFA) to a eukaryote for direct measurement of replication dynamics.

Conclusions:

The study provides a high-resolution resource for studying genome biology.
The developed methodological framework enables detailed analysis of eukaryotic DNA replication.
Findings suggest conserved replication origin usage and activity across different ploidy levels in yeast.