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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Binocular Dynamic Visual Acuity in Eyeglass-Corrected Myopic Patients
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The orthoptic slit lamp.

Marcus-Matthias Gellrich1

  • 1Ophthalmological Practice , Kellinghusen , Germany.

Strabismus
|October 5, 2013
PubMed
Summary

This study details modifications to slit lamp videography for documenting strabismus (squint). These adaptations enhance imaging of eye positions, aiding diagnosis of various eye alignment conditions.

Area of Science:

  • Ophthalmology
  • Medical Imaging

Background:

  • Slit lamp videography offers a comprehensive imaging solution for ophthalmic pathologies.
  • Documenting strabismus (squint) requires specific adaptations to standard slit lamp equipment.

Purpose of the Study:

  • To describe modifications for slit lamp videography specifically for documenting squint.
  • To enhance the visualization and recording of binocular eye positions in strabismus.

Main Methods:

  • Adjusting the observation system with minus lenses (-8 diopters) for binocular focus at 50 cm.
  • Modifying the illumination system using a 14 diopter recentration prism to optimize facial and corneal reflex illumination.
  • Utilizing digital recorders for capturing transient eye movements, crucial for diagnosing latent or intermittent strabismus.

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  • Employing Microsoft PowerPoint for creating composite images (9-gaze) and comparing eye positions through foil flickering.

    • Main Results:

    • Achieved sharp, simultaneous imaging of both eyes for strabismus documentation.
    • Ensured adequate illumination of the patient's face and central corneal reflexes.
    • Enabled identification of brief, dynamic changes in binocular eye positions.
    • Facilitated visualization of post-surgical or diagnostic cover test-induced eye position changes.

    Conclusions:

    • Modified slit lamp videography provides an effective method for documenting squint.
    • These technical adjustments improve the diagnostic capability of slit lamp imaging for strabismus.
    • The described techniques aid in the assessment of both static and dynamic aspects of eye alignment disorders.