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Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease
Published on: April 12, 2021
Single primary fetal lung cells generate alveolar structures in vitro
Shengliang Zhang1, Xin Zhou, Tie Chen
1Laboratory of Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China, 610041.
In Vitro Cellular & Developmental Biology. Animal
|October 5, 2013
Summary
Researchers developed a novel 3D culture system to study lung alveolar morphogenesis. This in vitro model mimics in vivo lung development, aiding the investigation of cellular and molecular mechanisms.
Area of Science:
- Developmental Biology
- Cell Biology
- Tissue Engineering
Background:
- Organ morphogenesis, including lung development, presents challenges for in vivo observation due to imaging limitations.
- In vitro models are crucial for studying cellular behaviors and developmental processes relevant to in vivo morphogenesis.
Purpose of the Study:
- To establish and validate a novel three-dimensional (3D) in vitro culture system for assessing mouse lung alveolar morphogenesis.
- To provide a tool for investigating the cellular and molecular mechanisms underlying lung development.
Main Methods:
- Primary fetal mouse lung cells were cultured in a 3D Matrigel matrix.
- The culture medium was supplemented with fibroblast growth factor 10 (FGF10) and hepatocyte growth factor (HGF).
Main Results:
- Single primary mouse fetal lung cells successfully proliferated and differentiated in the 3D culture.
- The cells formed lumen and developed into multivesicular epithelial structures.
- The resulting morphology closely resembled in vivo lung alveoli.
Conclusions:
- The developed 3D culture system effectively models mouse lung alveolar morphogenesis in vitro.
- This system serves as a valuable tool for studying the cellular and molecular underpinnings of lung development.
