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Sodium current in single myocardial mouse cells
Pflugers Archiv : European Journal of Physiology
|May 1, 1985
Summary
This study characterizes adult mouse myocardial cells, detailing their dimensions and electrical properties using voltage clamp techniques. Findings reveal specific membrane resistance and sodium current characteristics, crucial for understanding cardiac electrophysiology.
Area of Science:
- Cardiology
- Electrophysiology
- Cell Biology
Background:
- Adult mouse myocardial cells possess distinct morphological characteristics.
- Understanding the electrical properties of single cardiomyocytes is essential for cardiac research.
Purpose of the Study:
- To characterize the electrophysiological properties of isolated adult mouse myocardial cells.
- To determine key electrical parameters including membrane resistance, capacitance, and sodium current kinetics.
Main Methods:
- Isolation and morphological assessment of adult mouse myocardial cells.
- Voltage clamp technique using a one suction pipette method.
- Analysis of capacitive current time constant, series resistance, membrane resistance, and membrane capacity.
Main Results:
- Myocardial cells measured 132 x 21 x 10 microns, exhibiting a brick-like shape.
- Capacitive current time constant (tau) was 35 +/- 14 microseconds.
- Specific membrane resistance (Rm) was calculated at 1,134 omega cm2.
- Tetrodotoxin (TTX) block of sodium current followed 1:1 binding kinetics with a KD of 1.4 X 10(-6) M.
- Sodium current activation and inactivation time constants were determined across various voltages.
Conclusions:
- The study provides detailed electrophysiological parameters for adult mouse myocardial cells.
- The data contributes to a better understanding of cardiac ion channel function and cellular electrophysiology.
- The characterized properties are valuable for computational modeling and further research in cardiac physiology.