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Related Experiment Video

Updated: May 7, 2026

Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography

Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography

Published on: July 17, 2018

Visualizing compaction of polysomes in bacteria.

Nicolas Cougot¹, Anne-Elisabeth Molza¹, Jérémy Delesques¹

¹Team Translation and Folding, Université de Rennes 1, UMR CNRS 6290 IGDR, Campus de Beaulieu, 35042 Rennes Cedex, France.

Journal of Molecular Biology

|October 8, 2013

Summary

This summary is machine-generated.

Bacterial cells lacking rescue systems reorganize protein synthesis machinery. Ribosomes form compact polysomes, like hairpins, to maintain translation efficiency under stress.

Keywords:

3D BSA GST RT-PCR TEM bovine serum albumin electron tomography glutathione S-transferase mRNA polysomes reverse transcription PCR ribosome three dimensional tmRNA transfer-messenger RNA transmission electron microscopy

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Last Updated: May 7, 2026

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Analysis of Translation Initiation During Stress Conditions by Polysome Profiling

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Published on: May 19, 2014

Area of Science:

Molecular Biology
Cell Biology
Biophysics

Background:

Polysomes (polyribosomes) are complexes of mRNA and multiple ribosomes.
Bacterial polysome organization is known in vitro, but poorly understood in constrained cellular conditions.
Translational pauses can alter ribosome behavior and cellular physiology.

Purpose of the Study:

To investigate the spatial organization of bacterial polysomes under constrained conditions.
To understand how ribosome networks adapt when cellular rescue systems are absent or overwhelmed.
To analyze the supramolecular network of ribosomes after inducing translational pauses in Escherichia coli.

Main Methods:

Electron tomography
Template matching
Three-dimensional modeling
Overexpression of mRNA with a polyproline motif in Escherichia coli
Utilizing a strain lacking transfer-messenger RNA

Main Results:

Inducing translational pauses in a strain lacking transfer-messenger RNA significantly altered the ribosomal network.
Single ribosomes were replaced by large amounts of compacted polysomes.
These polysomes exhibited high organization, forming stacked hairpins and dimers of hairpins.

Conclusions:

Compacted and organized polysome structures, such as hairpins, may be a mechanism to maintain translation efficiency.
This spatial arrangement is crucial when cellular rescue systems are absent or overwhelmed.
The study reveals novel insights into bacterial ribosome organization under stress.