Human cytomegalovirus polypeptides stimulate neutralizing antibody in vivo

At least three human cytomegalovirus polypeptides are targets for virus neutralizing antibody; a single protein of 86,000 molecular weight (p86) and two coimmunoprecipitating proteins of 130,000 and 55,000 molecular weight (p130/55). These polypeptides have been isolated by immunoaffinity chromatography and tested for immunogenicity in guinea pigs. Neutralizing antibody was detected after immunization with both p86 and p130/55. Hyperimmune sera to p130/55, but not p86, were dependent upon guinea pig complement for virus neutralization.