Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interaction among agents that block end-plate depolarization competitively.

B E Waud, D R Waud

    Anesthesiology
    |July 1, 1985
    PubMed
    Summary

    This study investigated neuromuscular blocking agents, finding that while most combinations acted additively, pancuronium plus metocurine and metocurine plus gallamine showed potentiation beyond simple competitive models.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The Vulcan kidney.

    Trends in pharmacological sciences·1999
    Same author

    Gifts from industry: laundering money or supporting education?

    JAMA·1994
    Same author

    Captopril, enalapril, and quality of life.

    The New England journal of medicine·1993
    Same author

    Pharmaceutical promotions--a free lunch?

    The New England journal of medicine·1992
    Same author

    Quantal bioassay and stroke.

    Stroke·1992
    Same author

    A primer on "grind and bind".

    Anesthesia and analgesia·1988

    Area of Science:

    • Pharmacology
    • Neuroscience
    • Muscle Physiology

    Background:

    • Neuromuscular blocking agents are crucial in anesthesia and critical care.
    • Understanding their combined effects is vital for safe clinical application.
    • The classical competitive model predicts additive effects for antagonists.

    Purpose of the Study:

    • To assess if combinations of gallamine, metocurine, pancuronium, and tubocurarine follow the classical competitive model.
    • To investigate deviations from simple additivity in drug interactions at the neuromuscular junction.

    Main Methods:

    • Isolated guinea pig lumbrical muscles were used to study depolarization.
    • Carbachol was used to induce depolarization, recorded via the moving fluid electrode technique.
    • Dose-response data were analyzed using a split plot factorial design to account for experimental variations.

    Related Experiment Videos

    Main Results:

    • Four out of six drug pairings exhibited simple additive effects, consistent with competitive antagonism.
    • The combination of pancuronium plus metocurine showed 41% potentiation beyond additivity.
    • Metocurine plus gallamine demonstrated 21% potentiation, deviating from the classical model.

    Conclusions:

    • Some neuromuscular blocking agent combinations, specifically pancuronium-metocurine and metocurine-gallamine, do not adhere to the simple classical competitive model.
    • These findings suggest potential synergistic interactions that warrant further investigation into alternative receptor models.
    • The results have implications for optimizing drug combinations in clinical settings.