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Updated: May 7, 2026
RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
Published on: March 11, 2014
Colorimetric detection of human papilloma virus by double isothermal amplification
Stefano Persano1, Paola Valentini, Joong Hyun Kim
1Istituto Italiano di Tecnologia (IIT), Center for Bio-Molecular Nanotechnologies@UniLe, Via Barsanti - 73010 Arnesano, Lecce, Italy. pierpaolo.pompa@iit.it.
Abstract:
We developed a polymerase reaction free, low-cost and sensitive assay for the colorimetric detection of Human Papilloma Virus (HPV), based on the use of a smart design exploiting magnetic microbeads, chimeric RNA/DNAzyme oligonucleotides, and double signal amplification. This method allows obtaining a fast response with a detection limit of 10 pM, avoiding the amplification of the target via traditional PCR.
