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Updated: May 7, 2026
Establishing a Silicosis Rat Model via Exposure of Whole-Body to Respirable Silica
Published on: October 28, 2022
Protein expression in silica dust-induced transdifferentiated rats lung fibroblasts
Chang Fu Hao1, Xiao Fang Li, Wu Yao
1Department of Occupational Health and Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan, China.
Objective:
To analyze the expression of different proteins in free silica-induced transdifferentiated rat lung fibroblasts.
Methods:
Rat lung fibroblasts and alveolar macrophages were cultured. A transdifferentiation model of rat lung fibroblasts was established. Free silica was used as a stimulator for rat lung fibroblasts. Changes in α-SMA were detected by immunohistochemistry and Western blot, respectively. Protein of lung fibroblasts was extracted and analyzed by two-dimensional electrophoresis (2-DE).
Results:
Six protein spots were identified by mass spectrometry, including glyceraldehyde 3-phosphate-dehydrogenase, peroxiredoxin 5, heterogeneous nuclear ribonucleoprotein A2, transgelin 2, keratin K6 and vimentin.
Conclusion:
Some proteins are changed in free silica-induced transdifferentiated rat lung fibroblasts.
