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Transgene-induced pleiotropic effects in transplastomic plants
1Res. Div. Portici, Institute of Plant Genetics, National Research Council of Italy, CNR-IGV, via Università 133, 80055, Portici, NA, Italy, nscotti@unina.it.
Biotechnology Letters
|October 9, 2013
Summary
Plastid transformation enables high-level, stable production of recombinant proteins in plants. However, unintended effects on plant growth and physiology can occur, prompting the development of new technologies to address these challenges.
Area of Science:
- Plant Biotechnology
- Molecular Biology
- Genetics
Background:
- Plastid transformation allows stable transgene integration into the plant plastome.
- Transplastomic plants are effective for producing recombinant proteins with high yields and containment.
- Over 100 transgenes have been successfully expressed in plant chloroplasts.
Purpose of the Study:
- To review unintended pleiotropic effects observed in transplastomic plants.
- To discuss technologies developed to overcome these challenges in plastid transformation.
Main Methods:
- Literature review of studies on plastid transformation and its effects.
- Analysis of reported pleiotropic effects on plant growth and physiology.
- Examination of mitigation strategies and technologies.
Main Results:
- Plastid transformation offers advantages in protein production and transgene containment.
- Unintended pleiotropic effects on plant physiology and growth have been documented.
- Various technologies have been developed to manage or resolve these unintended effects.
Conclusions:
- Plastid transformation is a powerful tool for bioproduction, but careful consideration of potential side effects is necessary.
- Ongoing technological advancements aim to improve the safety and efficacy of transplastomic plants.
- Further research is needed to fully understand and mitigate pleiotropic effects for broader application.
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