Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flame Photometry: Lab01:16

Flame Photometry: Lab

1.3K
In a flame photometer, when a solution like potassium chloride is aspirated into the flame, the solvent evaporates, leaving behind dehydrated salt. This salt dissociates into free gaseous atoms in their ground state. Some of these atoms absorb energy from the flame, leading to their excitation. The excited atoms return to the ground state, emitting photons at characteristic wavelengths. Because only electronic transitions are involved, the resulting emission lines are very narrow. The intensity...
1.3K
Photoelectric Effect02:26

Photoelectric Effect

30.8K
When light of a particular wavelength strikes a metal surface, electrons are emitted. This is called the photoelectric effect. The minimum frequency of light that can cause such emission of electrons is called the threshold frequency, which is specific to the metal. Light with a frequency lower than the threshold frequency, even if it is of high intensity, cannot initiate the emission of electrons. However, when the frequency is higher than the threshold value, the number of electrons ejected...
30.8K
Light Acquisition02:16

Light Acquisition

8.0K
In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
8.0K
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

4.0K
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
4.0K
Photoluminescence: Applications01:14

Photoluminescence: Applications

1.3K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.3K
Flame Photometry: Overview01:02

Flame Photometry: Overview

2.1K
Flame photometry, also known as flame emission spectrometry, is a technique used for the qualitative and quantitative analysis of elements present in a sample using a flame as the source of excitation energy. The concept of flame photometry was realized in the early 1860s by Kirchhoff and Bunsen, who discovered that specific elements emit characteristic radiation when excited in flames. The first instrument developed for this purpose was used to measure sodium (Na) in plant ash using a Bunsen...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of Non-basic Matrix Domain Residues That Impact HTLV-1 Gag Membrane Targeting and Particle Release.

Journal of molecular biology·2026
Same author

Fluorescence Intensity of Protein Tags Is Dependent on Their Subcellular Location.

Cells·2026
Same author

3D localization of retrovirus assembly in the presence of structured background with deep learning.

Biophysical journal·2025
Same author

Validation of the Kidsights Measurement Tool: A parent-reported instrument to track children's development at the population level.

PloS one·2025
Same author

Quantifying glucose uptake at the single cell level with confocal microscopy reveals significant variability within and across individuals.

Scientific reports·2025
Same author

Identification of the GABARAP binding determinant in PI4K2A.

Bioscience reports·2024
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 7, 2026

Blue-hazard-free Candlelight OLED
10:18

Blue-hazard-free Candlelight OLED

Published on: March 19, 2017

9.0K

Brightness experiments.

Patrick J Macdonald1, Jolene Johnson, Yan Chen

  • 1Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 11, 2013
PubMed
Summary
This summary is machine-generated.

This study details quantitative fluorescence brightness experiments for analyzing protein interactions within living cells. It provides methods for accurate measurements, data interpretation, and determining protein binding and stoichiometry.

More Related Videos

Enabling High Grayscale Resolution Displays and Accurate Response Time Measurements on Conventional Computers
06:50

Enabling High Grayscale Resolution Displays and Accurate Response Time Measurements on Conventional Computers

Published on: February 29, 2012

8.8K
An Experimental Approach to Investigating Effects of Artificial Light at Night on Free-Ranging Animals: Implementation, Results, and Directions for Future Research
06:16

An Experimental Approach to Investigating Effects of Artificial Light at Night on Free-Ranging Animals: Implementation, Results, and Directions for Future Research

Published on: February 2, 2022

1.9K

Related Experiment Videos

Last Updated: May 7, 2026

Blue-hazard-free Candlelight OLED
10:18

Blue-hazard-free Candlelight OLED

Published on: March 19, 2017

9.0K
Enabling High Grayscale Resolution Displays and Accurate Response Time Measurements on Conventional Computers
06:50

Enabling High Grayscale Resolution Displays and Accurate Response Time Measurements on Conventional Computers

Published on: February 29, 2012

8.8K
An Experimental Approach to Investigating Effects of Artificial Light at Night on Free-Ranging Animals: Implementation, Results, and Directions for Future Research
06:16

An Experimental Approach to Investigating Effects of Artificial Light at Night on Free-Ranging Animals: Implementation, Results, and Directions for Future Research

Published on: February 2, 2022

1.9K

Area of Science:

  • Biophysics
  • Cell Biology
  • Biochemistry

Background:

  • Quantitative fluorescence brightness experiments are crucial for studying molecular interactions in living cells.
  • Accurate interpretation requires careful consideration of fluorescent labels, calibration, and potential experimental pitfalls.

Purpose of the Study:

  • To provide an overview of quantitative fluorescence brightness experiments, focusing on single-color measurements of protein homo-interactions.
  • To guide researchers on practical aspects, data interpretation, and advanced techniques like brightness titration.

Main Methods:

  • Discusses selection and calibration of fluorescent labels.
  • Addresses common issues like photobleaching, saturation, and undersampling.
  • Explains moment analysis and photon detector effect correction for brightness data.

Main Results:

  • Provides a framework for quantitative interpretation of fluorescence brightness data.
  • Outlines methods to mitigate experimental artifacts and improve data accuracy.
  • Demonstrates the utility of brightness titration for determining binding curves and stoichiometry.

Conclusions:

  • Quantitative fluorescence brightness assays offer a powerful method for characterizing protein interactions in vivo.
  • Proper experimental design and data analysis are essential for reliable results.
  • Brightness titration is a valuable technique for quantifying protein binding parameters.