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Suppression of the mixed leukocyte reaction by serum from polynucleotide-injected mice
Sera from mice which have been injected iv with either poly(A) X poly(U) or poly(I) X poly(C) 1 1/2 hr prior to bleeding were found to suppress the mixed lymphocyte reaction. This effect was reduced considerably by 18 hr. Characterization of the suppressive sera revealed it (a) was stable to heating at 56 degrees C for 1 hr and freezing at -20 degrees C for 1 month; (b) had a molecular weight greater than 30,000; (c) could be induced in sera from athymic nude mice; and (d) was present to a lower degree in sera from aging mice.
Sera from mice which have been injected iv with either poly(A) X poly(U) or poly(I) X poly(C) 1 1/2 hr prior to bleeding were found to suppress the mixed lymphocyte reaction. This effect was reduced considerably by 18 hr. Characterization of the suppressive sera revealed it (a) was stable to heating at 56 degrees C for 1 hr and freezing at -20 degrees C for 1 month; (b) had a molecular weight greater than 30,000; (c) could be induced in sera from athymic nude mice; and (d) was present to a lower degree in sera from aging mice.