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Related Experiment Videos

A method for removing stain precipitate and destaining sections.

W R Colquhoun, J A Lasher

    Stain Technology
    |May 1, 1985
    PubMed
    Summary
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    Sodium hydroxide treatment effectively removes stain precipitate and extracts stain from electron microscope sections. This allows for restaining to achieve cleaner images or obtain additional histochemical data from the same sections.

    Area of Science:

    • Electron microscopy
    • Histochemistry
    • Materials science

    Background:

    • Stain precipitate can obscure details in electron microscope images.
    • Existing methods for removing precipitate may damage tissue sections.
    • Sequential staining can provide complementary data but requires effective destaining.

    Purpose of the Study:

    • To evaluate the efficacy of sodium hydroxide (NaOH) treatment for removing stain precipitate from electron microscope sections.
    • To assess the impact of NaOH treatment on tissue integrity.
    • To determine the feasibility of restaining or sequential staining after NaOH treatment for enhanced histochemical analysis.

    Main Methods:

    • Electron microscope sections were subjected to sodium hydroxide (NaOH) treatment.

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  • Sections were analyzed for stain precipitate removal from the surface and within the tissue.
  • Sections were restained with the same or different stains after NaOH treatment and destaining.
  • Image quality and histochemical information were compared before and after treatment.

    • Main Results:

    • NaOH treatment effectively removed stain precipitate from the section surface.
    • Alkali treatment also extracted stain from within the tissue.
    • Restaining resulted in clean images, improving visualization.
    • Destained sections could be successfully restained with different stains, yielding additional histochemical information.

    Conclusions:

    • NaOH treatment is a viable method for removing stain precipitate in electron microscopy.
    • This technique allows for obtaining cleaner images and enables sequential histochemical analysis on the same sections.
    • The method preserves the integrity of the tissue, facilitating further investigation.