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Related Experiment Videos

Lymphoblastoid cell variant in contact-mediated cell spreading.

J A Sorrentino, A J Millis, A P Whipple

    Experimental Cell Research
    |May 1, 1983
    PubMed
    Summary

    Human lymphoblastoid cells exhibit contact-mediated cell spreading, a process dependent on a specific cell surface glycoconjugate. A variant clone lacking this ability can be restored by specific amino sugars.

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    Area of Science:

    • Cell Biology
    • Biochemistry

    Background:

    • Contact-mediated cell spreading is a fundamental cellular behavior.
    • Human lymphoblastoid cells offer a model for studying cell adhesion and spreading.

    Purpose of the Study:

    • To investigate the mechanism of contact-mediated cell spreading in human lymphoblastoid cells.
    • To identify the molecular components involved in this process.

    Main Methods:

    • Isolation and characterization of cell clones with differing spreading capabilities.
    • Transmission electron microscopy (TEM) and differential interference contrast microscopy for morphological analysis.
    • Biochemical analysis of cell surface components, including iodination and molecular weight shifts.

    Main Results:

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    • A clone of human lymphoblastoid cells capable of contact-mediated cell spreading was identified.
    • A variant clone lacking spreading ability was isolated; its adhesion could be restored by specific amino sugars (glucosamine or mannosamine).
    • Adhesion restoration correlated with a molecular weight shift in an iodinatable cell surface component.

    Conclusions:

    • Contact-mediated cell spreading in lymphoblastoid cells is mediated by a cell surface glycoconjugate.
    • Defective synthesis of this glycoconjugate underlies the non-adhesive phenotype in the variant clone.
    • Amino sugars play a crucial role in restoring the function of this glycoconjugate.