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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Related Experiment Video

Updated: May 7, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications
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Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon

D D-U Li, S Poland, S Coelho

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
    |October 11, 2013
    PubMed
    Summary
    This summary is machine-generated.

    A new fast, hardware-friendly algorithm for bi-exponential fluorescence lifetime analysis is presented, optimized for 2D CMOS single-photon avalanche diode (SPAD) arrays. Its performance was validated using multi-photon microscopy on a plant specimen.

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    Area of Science:

    • Biophysics
    • Microscopy
    • Photonics

    Background:

    • Fluorescence lifetime imaging microscopy (FLIM) is crucial for biological research.
    • Existing FLIM algorithms can be computationally intensive and not hardware-friendly.
    • Advanced imaging systems require efficient data processing techniques.

    Purpose of the Study:

    • To develop a novel, hardware-friendly algorithm for bi-exponential fluorescence lifetime analysis.
    • To optimize the algorithm for 2D CMOS single-photon avalanche diode (SPAD) arrays.
    • To demonstrate the algorithm's performance and efficiency in a practical biological application.

    Main Methods:

    • Development of a fast, hardware-optimized bi-exponential decay fitting algorithm.
    • Implementation and testing on 0.13µm CMOS SPAD arrays.
    • Acquisition of multi-photon microscopy data from a plant specimen (Convallaria).

    Main Results:

    • The proposed algorithm offers a significant speed advantage while maintaining accuracy.
    • Successful application of the algorithm to complex biological samples.
    • Demonstrated suitability for real-time or near-real-time analysis in advanced microscopy systems.

    Conclusions:

    • The developed algorithm is an efficient and practical solution for bi-exponential fluorescence lifetime analysis.
    • It enhances the capabilities of 2D CMOS SPAD-based multi-photon microscopy.
    • This advancement facilitates more detailed investigations of biological processes using FLIM.