Beading of the astrocytic processes (clasmatodendrosis) following head trauma is associated with protein degradation pathways
Kentaro Sakai1, Takahiro Fukuda, Kimiharu Iwadate
1Department of Forensic Medicine , and.
Objectives:
To present the incidence of clasmatodendrosis (injured astrocytic processes) and analyse the association between clasmatodendrosis and the ubiquitin proteasome system (UPS) and autophagy lysosome system (autophagy).
Methods:
The injured cerebral cortices of 36 autopsy cases were analysed by light microscopy by immunohistochemistry using an anti-glial fibrillary acidic protein (GFAP) antibody and UPS marker antibodies (ubiquitin and lysine 48-linked polyubiquitin chains (K(48)) and autophagy marker (lysine 63-linked polyubiquitin chains (K(63)), p62/sequestosome 1 and microtubule-associated protein 1 light chain 3 [LC3]). Double-immunofluorescence staining was used to detect the relationships between p62-K(48), p62-K(63) and GFAP-K(48).
Results:
Clasmatodendrosis that was immunoreactive to GFAP or K(48) was detected by 1 hour up to 14 days following head trauma. The survival time of cases with clasmatodendrosis was significantly shorter than that in cases without clasmatodendrosis (p < 0.01). Involvement of contusion or oedema was significantly frequent in cases with clasmatodendrosis (p < 0.01). There was no significant age difference between cases with clasmatodendrosis and cases without clasmatodendrosis (p = 0.18). Double-immunofluorescence staining for p62-K(48) and GFAP-K(48) demonstrated clasmatodendrosis with overlapping fluorescence signals.
Conclusion:
Clasmatodendrosis is associated with UPS-mediated, autophagy and relatively acute pathological findings after traumatic intracranial injury.
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