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Exposures to molds in school classrooms of children with asthma
Sachin N Baxi1, Michael L Muilenberg, Christine A Rogers
1Division of Allergy and Immunology, Department of Pediatrics, Boston Children's Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USA.
Background:
Students spend a large portion of their day in classrooms which may be a source of mold exposure. We examined the diversity and concentrations of molds in inner-city schools and described differences between classrooms within the same school.
Methods:
Classroom airborne mold spores, collected over a 2 day period, were measured twice during the school year by direct microscopy.
Results:
There were 180 classroom air samples collected from 12 schools. Mold was present in 100% of classrooms. Classrooms within the same school had differing mold levels and mold diversity scores. The total mold per classroom was 176.6 ± 4.2 spores/m3 (geometric mean ± standard deviation) and ranged from 11.2 to 16,288.5 spores/m3. Mold diversity scores for classroom samples ranged from 1 to 19 (7.7 ± 3.5). The classroom accounted for the majority of variance (62%) in the total mold count, and for the majority of variance (56%) in the mold diversity score versus the school. The species with the highest concentrations and found most commonly included Cladosporium (29.3 ± 4.2 spores/m3), Penicillium/Aspergillus (15.0 ± 5.4 spores/m3), smut spores (12.6 ± 4.0 spores/m3), and basidiospores (6.6 ± 7.1 spores/m3).
Conclusions:
Our study found that the school is a source of mold exposure, but particularly the classroom microenvironment varies in quantity of spores and species among classrooms within the same school. We also verified that visible mold may be a predictor for higher mold spore counts. Further studies are needed to determine the clinical significance of mold exposure relative to asthma morbidity in sensitized and non-sensitized asthmatic children.
Insights
Classroom mold levels vary significantly within schools, with the classroom environment being a major factor in spore concentration and diversity. Visible mold can indicate higher spore counts, warranting further study into health impacts.
Area of Science:
- Environmental Science
- Indoor Air Quality
- Microbiology
Background:
- Students spend significant time in classrooms, potentially leading to mold exposure.
- Inner-city schools were assessed for airborne mold diversity and concentrations.
Purpose of the Study:
- To examine mold diversity and concentrations in inner-city school classrooms.
- To identify variations in mold levels and species between classrooms within the same school.
Main Methods:
- Collected airborne mold spore samples from 180 classrooms across 12 schools over a 2-day period, twice during the school year.
- Utilized direct microscopy for spore analysis.
Main Results:
- Mold was detected in 100% of classrooms, with significant variations in mold levels and diversity scores between classrooms in the same school.
- The classroom environment accounted for the majority of variance in total mold count (62%) and mold diversity (56%).
- Common mold species included Cladosporium, Penicillium/Aspergillus, smut spores, and basidiospores.
Conclusions:
- Schools are a source of mold exposure, with significant microenvironmental variations in mold quantity and species within classrooms.
- Visible mold may predict higher airborne mold spore counts.
- Further research is needed to link mold exposure to asthma morbidity in children.
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