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Eicosanoid post-mortem induction in kidney tissue is prevented by microwave irradiation
Stephen A Brose1, Mikhail Y Golovko
1Department of Pharmacology, Physiology, and Therapeutics, University of North Dakota, 501 N. Columbia Road, Grand Forks, ND 58202-9037, USA.
Prostaglandins, Leukotrienes, and Essential Fatty Acids
|October 12, 2013
Summary
Post-mortem analysis reveals significant increases in kidney eicosanoids without microwave fixation, highlighting the critical need for tissue fixation in accurate analysis.
Area of Science:
- Biochemistry
- Physiology
- Analytical Chemistry
Background:
- Prostanoid (PG) levels increase post-mortem in brain tissue without microwave fixation.
- This increase is not due to PG trapping or destruction by microwave irradiation.
Purpose of the Study:
- To investigate post-mortem changes in kidney eicosanoid levels.
- To determine the impact of tissue fixation on kidney eicosanoid analysis.
Main Methods:
- Analysis of kidney eicosanoids (prostaglandins, HETEs, arachidonic acid) with and without microwave fixation.
- Inhibition of cyclooxygenase with indomethacin to assess prostaglandin synthesis.
Main Results:
- Dramatic increases in medullary 6-ketoPGF1α, PGE2, and PGF2α (142-, 81-, 62-fold) were observed in non-microwaved kidney tissue.
- Significant increases in whole kidney and cortex PG, as well as hydroxyeicosatetraenoic acids (HETEs) and arachidonic acid, were noted in non-microwaved samples.
- Indomethacin reduced PG levels but did not affect HETE or arachidonic acid levels in non-microwaved tissue.
Conclusions:
- Kidney tissue fixation is critical to prevent artifactual increases in eicosanoid levels during analysis.
- Post-mortem changes significantly impact kidney eicosanoid measurements, emphasizing the need for proper fixation techniques.
