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A monoclonal lambda 1-bearing anti-dextran antibody from a lambda-defective mouse strain
A hybridoma from the lambda-defective mouse strain SJA has been established. It produces a lambda 1-bearing IgG2b, dextran B 1355-binding antibody. The DNA sequence of the VJ and C gene segment was in complete accordance with the published germ-line sequence. The rate of secretion and the steady state level of cytoplasmic RNA of this line was comparable to that of cell lines from normal mice. The idiotype was closely related to that of MOPC 104E indicating that the lambda 1 light chain is associated with the same VH region and in a similar fashion as in BALB/c mice. This antibody should be useful for further experiments on the lambda defect of SJL or SJA mice.
A hybridoma from the lambda-defective mouse strain SJA has been established. It produces a lambda 1-bearing IgG2b, dextran B 1355-binding antibody. The DNA sequence of the VJ and C gene segment was in complete accordance with the published germ-line sequence. The rate of secretion and the steady state level of cytoplasmic RNA of this line was comparable to that of cell lines from normal mice. The idiotype was closely related to that of MOPC 104E indicating that the lambda 1 light chain is associated with the same VH region and in a similar fashion as in BALB/c mice. This antibody should be useful for further experiments on the lambda defect of SJL or SJA mice.