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Related Experiment Videos

A monoclonal lambda 1-bearing anti-dextran antibody from a lambda-defective mouse strain.

S Weiss, K Lehmann, M Cohn

    European Journal of Immunology
    |August 1, 1985
    PubMed
    Summary

    A new hybridoma producing a specific antibody was created from a lambda-defective mouse. This antibody, useful for studying lambda defects, shows characteristics similar to those in normal mice.

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    Area of Science:

    • Immunology
    • Molecular Biology

    Background:

    • Hybridomas are crucial for producing monoclonal antibodies.
    • Understanding antibody production in genetically modified mouse strains is important for research.

    Purpose of the Study:

    • To establish a hybridoma from the SJA mouse strain, which has a lambda-defective genetic makeup.
    • To characterize the antibody produced by this hybridoma for its potential use in studying lambda defects.

    Main Methods:

    • Hybridoma technology was used to establish a cell line from the SJA mouse strain.
    • DNA sequencing was performed to analyze the VJ and C gene segments.
    • Antibody secretion rates and cytoplasmic RNA levels were measured.
    • Idiotype analysis was conducted to compare with known antibodies.

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    Main Results:

    • A hybridoma producing a lambda 1-bearing IgG2b antibody that binds dextran B 1355 was successfully established.
    • The DNA sequence of the antibody's gene segments matched the germ-line sequence.
    • The antibody secretion rate and RNA levels were comparable to those of cell lines from normal mice.
    • The antibody's idiotype was closely related to MOPC 104E, suggesting similar VH region association.

    Conclusions:

    • The established hybridoma provides a valuable tool for investigating the lambda defect in SJA and SJL mice.
    • The antibody's characteristics suggest normal heavy and light chain association despite the lambda defect.
    • This research contributes to understanding antibody gene expression and potential therapeutic applications.