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Expression and function of Allergin-1 on human primary mast cells
Kei Nagai1, Satoko Tahara-Hanaoka, Yuko Morishima
1Department of Immunology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan ; Department of Nephrology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan ; Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Tokyo, Japan.
Plos One
|October 12, 2013
Summary
Allergin-1S1, a key inhibitory receptor, is predominantly found on human mast cells (MC). This receptor suppresses IgE-mediated MC activation, similar to its function in mice.
Area of Science:
- Immunology
- Cell Biology
Background:
- Mast cells (MC) are crucial in allergic and non-allergic immune responses.
- Cell surface inhibitory receptors modulate human MC activation.
- Allergin-1, a recently identified receptor, is present on human and mouse MC.
Purpose of the Study:
- To determine the expression profile and function of Allergin-1 on human primary MC.
- To establish a method for assessing human primary MC with limited cell numbers.
Main Methods:
- Developed a seven-color flow cytometry technique.
- Analyzed human primary MC from bronchoalveolar lavage (BAL) fluid and nasal scrapings.
- Assessed IgE-mediated activation of human primary MC.
Main Results:
- Identified Allergin-1S1 as the predominant splicing isoform of Allergin-1 on human primary MC.
- Demonstrated that Allergin-1S1 expression is significant in BAL fluid and nasal specimens.
- Showed Allergin-1S1 inhibits IgE-mediated activation of human primary MC from BAL fluid.
Conclusions:
- Human primary MC express Allergin-1S1, a functional inhibitory receptor.
- Allergin-1 on human MC shares similar expression and function with its mouse counterpart.
- Allergin-1S1 represents a potential therapeutic target for allergic diseases.
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